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Example of indirect immunofluorescence. Whole-mount indirect immunofluorescence was performed with Nv-NF-κB-specific antiserum on a 4-week-old Nematostella polyp. Nv-NF-κB was detected with FITC-conjugated secondary antiserum. (a) Without AR. (b) With AR using citrate buffer. (c) With AR using urea. All images were taken using a FluoView FV10i confocal microscope with approximately the same settings for FITC (green) detection.
