Fig. 1.

Bacteria identification to the species level by broad-range 16S rDNA PCR and sequencing from 19 samples with negative culture. This general tree was generated using the neighbour-joining (NJ) with the Tamura–Nei model. The numbers shown at the branch point indicate bootstrap values. The dataset was subjected to 1000 bootstrap replications. The sequences were selected from the GenBank database, and their accession numbers are listed in brackets. Sequences with an asterisk correspond with the clinical samples from this study, and the sequence accession numbers are GenBank KJ437454 to KJ437472. All the sequences could be an assign unambiguously to a genus. The species designation was determining with the closest genetic identification based on GenBank sequence analysis