Figure 2.

GPER activation enhances OR and OP memory. A, Mice receiving DH infusion of 4 ng/hemisphere G-1 (but not vehicle or 2 ng/hemisphere G-1) spent more time than chance (dashed line at 15 s) with the novel object 48 h after training. This group also spent more time with the novel object than vehicle, indicating enhanced memory for the familiar object (vehicle, n = 11; 2 ng G-1, n = 11; 4 ng G-1, n = 11). B, Similarly, mice infused with 4 ng G-1, but not vehicle or 2 ng G-1, spent significantly more time with the moved object than the vehicle group or than chance 24 h after OP training, indicating enhanced spatial memory (vehicle, n = 11; 2 ng G-1, n = 9; 4 ng G-1, n = 10). C, Mice receiving 7.4 ng G-15 exhibited impaired OR memory relative to vehicle and chance 24 h after DH infusion, whereas mice receiving vehicle, 1.85 ng G-15, or 3.7 ng G-15 did not (vehicle, n = 13; 1.85 ng G-15, n = 10; 3.7 ng G-15, n = 11; 7.4 ng G-15, n = 10). D, In OP, 7.4 ng G-15 impaired spatial memory relative to vehicle and chance 4 h after DH infusion, but no other dose of G-15 affected memory (vehicle, n = 16; 1.85 ng G-15, n = 13; 3.7 ng G-15, n = 13; 7.4 ng G-15, n = 14). E, Intracerebroventricular infusion of 8 ng G-1 significantly enhanced 48 h OR relative to vehicle (Veh) and chance, and DH infusion of 1.85 ng G-15 abolished this effect (Veh+Veh, n = 12; G-1+Veh, n = 11; G-1+G-15, n = 10). F, Similarly, G-15 prevented G-1 from enhancing OP relative to vehicle and chance (Veh+Veh, n = 15; G-1+Veh, n = 13; G-1+G-15, n = 12). Each bar represents the mean ± SEM time spent with the novel or moved object (*p < 0.05, **p < 0.01,***p < 0.001 relative to chance; #p < 0.05, ##p < 0.01 relative to vehicle). n.s., Non-significant.