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. 2016 Mar 10;7:10937. doi: 10.1038/ncomms10937

Figure 2. The top strand of attI1 is reactive.

Figure 2

(a) Experimental set-up used for mismatched-covalent-circles recombination. Mismatched circles bearing an attI1 site are transformed in an E. coli MG1655 mutS− strain bearing a pSU-attI1 plasmid and expressing the integrase in trans. (b) Representation of the recombination products resulting from the three possible HJ resolution pathways. The mismatch-bearing regions were PCR amplified and subjected to NarI and SacII restriction. (c) Restriction pattern analysis of three clones obtained, representing the three resolution pathways. (d) Sequencing of clone 36 PCR product revealed the expected mismatches.