Table 1.
Real-time PCR primer sequences.
| Gene | Primer sequence | |
|---|---|---|
| IL-1β | Forward | 5′-CCCTGCAGCTGGAGAGTGTGG-3′ |
| Reverse | 5′-TGTGCTCTGCTTGAGAGGTGCT-3′ | |
|
| ||
| IL-6 | Forward | 5′-TTCCTACCCCAACTTCCAATG-3′ |
| Reverse | 5′-ATGAGTTGGATGGTCTTGGTC-3′ | |
|
| ||
| TNF-α | Forward | 5′-GACCCTCACACTCAGATCATCTTCT-3′ |
| Reverse | 5′-TGCTACGACGTGGGCTACG-3′ | |
|
| ||
| NOS-II | Forward | 5′-CTTTACGCCACTAACAGTGGCA-3′ |
| Reverse | 5′-AGTCATGCTTCCCATCGCTC-3′ | |
|
| ||
| COX-2 | Forward | 5′-TGGTGCCGGGTCTGATGATG-3′ |
| Reverse | 5′-GCAATGCGGTTCTGATACTG-3′ | |
|
| ||
| GAPDH | Probe | Applied Biosystems® Rat GAPD (GAPDH) Endogenous Control (VIC®/MGB Probe, 4352338E) |