Table 2.
Viral and cellular genes expression levels in response to H3N2 influenza virus of A549 and MDCK cells evaluated by quantitative RT-PCR at the indicated time point. Ct values were determined for caspases 3, 8, and 9, TNFα, IκBα, BAD, MxA, and β-actin and GPDH genes.
| H3N2 | IκBα | BAD | TNF | Caspase 9 | Caspase 8 | Caspase 3 | MXA | β-actin | GPDH | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| MOI 0.1 | MOI 1.0 | MOI 0.1 | MOI 1.0 | MOI 0.1 | MOI 1.0 | MOI 0.1 | MOI 1.0 | MOI 0.1 | MOI 1.0 | MOI 0.1 | MOI 1.0 | MOI 0.1 | MOI 1.0 | MOI 0.1 | MOI 1.0 | MOI 0.1 | MOI 1.0 | |
| MDCK | ||||||||||||||||||
| 16 hpi | 22.46 | 22.76 | 16.38 | 15.85 | 25 | 25.8 | 24.2 | 25.3 | 25 | 26 | 26 | 276 | 27 | 26 | 17.2 | 17.2 | 22.2 | 22.2 |
| 24 hpi | 26.57 | 26.33 | 19.77 | 20.98 | 28.4 | 28.79 | 27.6 | 28.4 | 29 | 30 | 31.2 | 30.8 | 28 | 29 | 17.5 | 17.6 | 23.6 | 23.6 |
| 36 hpi | 24.82 | 25.2 | 18.4 | 19.9 | 26.72 | 26.99 | 26.4 | 27.0 | 27 | 27 | 28.7 | 27.4 | 26 | 28 | 17.9 | 18 | 23.2 | 23.2 |
| 48 hpi | 23.2 | 23.22 | 24.66 | 27.75 | 25.9 | 25.9 | 25.69 | 27 | 26 | 26 | 26.9 | 26.4 | 26 | 26 | 18.3 | 18.4 | 23 | 23 |
| A549 | ||||||||||||||||||
| 16 hpi | 19.2 | 19.69 | 16.8 | 18.4 | 20 | 20.9 | 22.8 | 21.9 | 19 | 20.6 | 21 | 21.6 | 27 | 27 | 16.9 | 16.9 | 20.3 | 20.6 |
| 24 hpi | 22.63 | 22.75 | 17.55 | 17.66 | 23 | 22.7 | 23 | 22.7 | 23.2 | 24.1 | 25.2 | 25.2 | 31 | 31.66 | 17.2 | 17.2 | 21.0 | 21.0 |
| 36 hpi | 21.25 | 22.25 | 18.22 | 22.5 | 24 | 22.9 | 24 | 23.6 | 21.65 | 21.45 | 23 | 23 | 29.4 | 29.88 | 17.9 | 17.8 | 21.0 | 21.0 |
| 48 hpi | 19.36 | 20.15 | 17.2 | 17.95 | 22 | 21.4 | 21.77 | 22.2 | 21 | 19.8 | 21.9 | 21.88 | 27.4 | 27.89 | 17.2 | 17.0 | 21.0 | 21.0 |