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. 2015 Oct 21;41(5):1308–1318. doi: 10.1038/npp.2015.280

Figure 3.

Figure 3

Effect of FR on CB1R receptor and BDNF expression in the hippocampus. Protein extracts, prepared from the hippocampus of CTRL and FR rats at the indicated times relative to food presentation, were subjected to immunoblot analysis with antibodies to CB1R or BDNF, and to GAPDH. (a, b) Representative blot as well as quantitation of the CB1R/GAPDH ratio (means±SEM from 5 to 10 rats) are shown. *P<0.001 vs. CTRL rats, one-way ANOVA with Bonferroni's post hoc test. (c, d) Representative blot as well as quantitation of the BDNF/GAPDH ratio (means±SEM from 5 to 10 rats) are shown. *P<0.001 vs CTRL rats, one-way ANOVA with Bonferroni's post hoc test. BDNF, brain-derived neurotrophic factor; CB1R, cannabinoid type-1 receptor; CTRL, control group; FR, food restriction.