Figure 3.

The anxiolytic-like effects of JZL184 are CB₁ dependent. (a–c) Intraperitoneal injection of rimonabant 17.5 h after TMT exposure causes no significant behavioral changes. (a) Ratio of time spent in open arm, (b) ratio of open-arm entry, and (c) anxiety index indicate that rimonabant (1 mg/kg) administration 18 h after TMT exposure has no effect on EPM behavior 7 days later (n=5–6 rats per group). (d–f) Intraperitoneal injection of rimonabant 30 min before a single JZL184 injection 18 h after TMT exposure blocks the anxiolytic-like effect of JZL184 that lasts for at least 7 days. (d) Ratio of time spent in open arm, (e) ratio of open-arm entry, and (f) anxiety index indicate that the anxiolytic-like effects of JZL184 are CB₁ dependent (n=11–12 rats per group). Results are expressed as mean ±SEM; *P<0.05, **P<0.01. CB₁, cannabinoid receptor 1; EPM, elevated plus maze; TMT, 2,5-dihydro-2,4,5-trimethylthiazoline.