The anxiolytic-like effects of MGL inhibition on TMT-induced anxiety-like behavior are mediated by the BLA. (a–c) Infusion of NF1819 into the BLA 18 h after TMT exposure has an anxiolytic-like effect that lasts for at least 7 days. (a) Ratio of time spent in open arm, (b) ratio of open-arm entry, and (c) anxiety index suggest that the anxiolytic-like effects of the MGL inhibitor occur in the BLA. (d–f) Infusion of NF1819 into the third ventricle has no overt behavioral effect in TMT-exposed rats. (d) Ratio of time spent in open arm, (e) ratio of open-arm entry, and (f) anxiety index (n=5 per group). Results are expressed as mean±SEM; n=6–10 rats per group; *P<0.05, **P<0.01. BLA, basolateral amygdala; MGL, monoacylglycerol lipase; TMT, 2,5-dihydro-2,4,5-trimethylthiazoline.