Figure 1.

Nfix Is Expressed by SCs and Its Absence Leads to a Reduced Myofiber Cross Sectional Area and to an Increased Slow MyHC Expression

(A) Immunofluorescence analysis of Nfix expression (green) in Pax7⁺ SCs (red) on a Tibialis anterior muscle section of a Mlc3f-lacz mouse, where myonuclei are in purple (β-gal). Hoechst was used to stain nuclei (n = 4 mice). The scale bar represents 25 μm.

(B) H&E staining on Tibialis anterior muscles from WT and Nfix-null mice (n = 5 mice). The scale bar represents 100 μm.

(C) Graphical representation of the myofiber cross sectional area (CSA) in WT and Nfix-null muscles. The plotted values represent the distribution of n = 65 measurements on five random microscope fields (^∗∗∗p < 0.001 and two-tailed unpaired t test). The data are presented as mean ± whiskers from min to max.

(D) Western blot analysis of slow MyHC expression in WT and Nfix-null Soleus (SOL) and EDL muscles. The β-tubulin was used to normalize.

See also Figure S1.