Figure 2.

SCs Lacking Nfix Are Characterized by a Delayed Differentiation

(A) Immunofluorescence analysis of MyoD (green) and Myogenin (Myog, red) expression on single muscle fibers from WT and Nfix-null muscles after 24, 48, 72, and 96 hr in culture. Hoechst was used to stain nuclei (n = 3 WT and 3 Nfix-null mice). The scale bar represents 50 μm.

(B) Quantification of MyoD⁺/Myogenin⁺ and MyoD⁺/Myogenin⁻ SCs associated with WT and Nfix-null myofibers at 24, 48, 72, and 96 hr in culture. The quantification is the result of three independent experiments on three WT and three Nfix-null mice. The data are presented as mean ± SD (not significant, ns; ^∗∗∗p < 0.001 with n = 11 WT myofibers and n = 16 Nfix-null myofibers; and two-tailed unpaired t test).

See also Figure S2.