Figure 3.

Absence of Nfix Leads to a Marked Impairment of Muscle Regeneration after Injury

(A) Immunofluorescence for dMHC (green) on regenerating WT and Nfix-null Tibialis anterior muscles 5, 7, 10, 14, and 28 days (d) following CTX injection. The laminin is marked in red and Hoechst was used to stain nuclei (n = 3 mice for each group). The scale bar represents 50 μm.

(B) Western blot analysis of Myogenin and Myostatin expression on WT and Nfix-null muscles 3, 5, 10, and 28 days (d) following CTX injection. The β-tubulin was used to normalize.

(C) Western blot analysis of pSmad3 and Smad3 expression on WT and Nfix-null muscles 5 and 7 days (d) following CTX injection. GAPDH was used to normalize.

(D) Quantification of the percentage of dMHC positive myofibers in WT and Nfix-null mice after 7 and 14 days following CTX injection (n = 6 mice for time point 7 and n = 5 mice for time point 14). The data are presented as mean ± SD (^∗p < 0.05; ^∗∗p < 0.01; and two-tailed unpaired t test).

See also Figure S2E.