Figure 4.

Absence of Nfix in SCs Determines the Delay in Muscle Regeneration

(A) H&E staining and immunofluorescence for dMHC (green) on transverse sections of regenerating Tibialis anterior muscles from Tg:Pax7-Cre^ERT2:Nfix^+/+ and Tg:Pax7-Cre^ERT2:Nfix^fl/−mice with (TMX) or without (NO TMX) tamoxifen treatment. The muscles were collected and stained after 5, 7, and 14 days (d) from CTX injection. The laminin is marked in red and Hoechst was used to stain nuclei (n = 3 mice for each group). The scale bar represents 100 μm.

(B) Western blot analysis of Myogenin expression on regenerating muscles from Tg:Pax7-Cre^ERT2:Nfix^+/+ (Nfix^+/+) and Tg:Pax7-Cre^ERT2:Nfix^fl/− mice (Nfix^fl/−) with (TMX) or without (NO TMX) tamoxifen treatment after 3, 5, 7, and 14 days (d) from CTX injection. Myogenin and GAPDH in the right panel come from two separate gels but using the same samples and protein amount. GAPDH was used to normalize.

See also Figure S3.