Figure 6.

In Vivo Silencing of Myostatin in Regenerating Nfix Null Muscles Rescues their Regeneration Defects

(A) Immunofluorescence analysis of dMHC expression (green) on regenerating WT and Nfix-null Tibialis anterior muscles after muscle electroporation with a control plasmid (scramble) or with a plasmid carrying an shRNA targeting Myostatin (shmstn). The muscles were electroporated after 4 days following CTX injection and were collected and stained after 7, 10, and 14 days (d) following CTX injection. The laminin is marked in red and Hoechst was used to stain nuclei (n = 3 mice for each group). The scale bar represents 100 μm.

(B) Quantification of the percentage of dMHC positive myofibers in Nfix-null mice electroporated with scramble or shmstn plasmids after 7 and 14 days following CTX injection (n = 5 mice for each group). The data are presented as mean ± SD (^∗p < 0.05 and two-tailed unpaired t test).

(C) Real-time qPCR showing Myostatin expression in myoblasts re-isolated from Tibialis anterior electroporated with control (scramble) or shMyostatin (shMstn) plasmids. The myoblasts were isolated the day after in vivo transfection, and cultures were stopped 2 days later to perform qRT-PCR. The data are presented as mean ± SD.

See also Figure S4.