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. Author manuscript; available in PMC: 2017 Jan 13.
Published in final edited form as: Cell Host Microbe. 2016 Jan 13;19(1):55–66. doi: 10.1016/j.chom.2015.12.004

Figure 5. Treatment of H. pylori cells with N-Acetylcysteine Blocks BabA Adherence.

Figure 5

(A) FITC-labeled 17875/Leb bacteria binding to human gastric tissue sections. Prior to binding, bacteria were preincubated with 0, 10, or 20 mg/mL NAC (i, ii, and iii, respectively), resulting in 100%, 14%, and less than 5% adherent bacteria, respectively. In panels iv, v, and vi, tissue sections with bound bacteria are treated with 10, 20, or 200 mg/mL NAC, resulting in undetectable, 50%, and over 90% bacterial detachment, respectively.

(B) Normalized RIA Leb-binding of H. pylori strain 17875/Leb when being subjected to increasing concentrations of N-acetylcysteine (0, 10, 20, 30, and 50 mg/mL) for 1 hr at 37°C. Data points show mean ± SD, n = 3.

(C and D) H. pylori epithelial adherence and neutrophil recruitment in gastric epithelium of mice treated for 2 weeks with 0 (−) or 40 (+) mg/day NAC in their drinking water. Data points show, per animal, mean bacterial counts (n = 3) per mm of immunostained gastric epithelium (C; Figure S5D), or mean neutrophil counts (n = 3) per mm2 of immunohistostained gastric sections (D; Figure S5E). Statistical comparison of the groups produced a Welch-corrected t(9) = 2.29, *p = 0.0475; and t(7) = 7.559, ***p = 0.0001, respectively. Horizontal lines show sample mean ± SD, n = 8 (−NAC) and 9 (+NAC). See also Figure S5.