Table 2. Levels of 8-oxo-dG and 8-oxo-dA in human retinal DNA.
| Donor # |
RPE Mitochondria |
NR Mitochondria |
NR Nuclei |
||||||
|---|---|---|---|---|---|---|---|---|---|
| DNA (μg) |
Adducts/106
nucleotides |
DNA (μg) |
Adducts/106
nucleotides |
DNA (μg) |
Adducts/106
nucleotides |
||||
| 8-oxo-dG | 8-oxo-dA | 8-oxo-dG | 8-oxo-dA | 8-oxo-dG | 8-oxo-dA | ||||
| Controls | |||||||||
| 1 | 0.031 | 329 | 22.3 | 0.010 | 194 | 7.94 | 22.4 | 0.66 | 0.07 |
| 2 | 0.037 | 226 | 9.41 | 0.115 | 19.9 | 0.98 | 20.3 | 0.67 | 0.05 |
| 3 | 0.011 | 405 | <LOQ | 0.020 | 69.4 | 17.9 | 4.82 | 0.63 | <LOQ |
| 4 | 0.055 | 137 | 15.6 | 0.182 | 52.3 | 9.02 | 2.96 | 0.54 | <LOQ |
| 5 | 0.051 | 207 | 16.4 | 0.092 | 58.3 | 8.83 | 31.0 | 0.18 | <LOQ |
| Donors with AMD | |||||||||
| 1 | 0.053 | 208 | 18.1 | 0.094 | 88.6 | 6.44 | 26.8 | 0.49 | 0.06 |
| 2 | 0.033 | 231 | 22.0 | 0.108 | 142 | 19.4 | 23.1 | 0.43 | 0.04 |
| 3 | 0.026 | 241 | 24.0 | 0.135 | 80.9 | 8.90 | 28.5 | 0.45 | 0.03 |
| 4 | 0.047 | 215 | 11.9 | 0.063 | 71.5 | 7.51 | 1.44 | 3.37 | <LOQ |
| 5 | 0.022 | 553 | 46.0 | 0.094 | 52.6 | 5.59 | 44.1 | 0.08 | 0.02 |
| Averagea | 0.037 | 275 | 18.6 | 0.091 | 83.0 | 9.24 | 20.5 | 0.75 | 0.045 |
| SD | 0.015 | 122 | 11.9 | 0.051 | 50.3 | 5.49 | 13.7 | 0.94 | 0.045 |
aAverage values are for controls and donors with AMD combined, because there was no statistically significant difference in DNA adduct levels between the two groups. To calculate average values, 1/2 of LOQ (0.8 fmol/sample) was used for samples in which 8-oxo-dA was not quantifiable. For each sample, LOQ was divided by DNA yield, and half of that value was used.