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. 2016 Apr;22(4):506–517. doi: 10.1261/rna.054445.115

FIGURE 2.

FIGURE 2.

(A) Time course of TAR RNA annealing with cTAR DNA in the presence or absence of various concentrations of NC. Lane 1, heat-denatured TAR 32P-RNA. TAR 32P-RNA alone (lanes 2) or mixed with cTAR DNA (lanes 3–12) was incubated at 20°C for 10 min (lanes 2,3,8), 1 h (lanes 4,9), 3 h (lanes 5,10), 6 h (lanes 6,11), or 24 h (lanes 7,12). The monomeric form of TAR is indicated by TAR*. The TAR RNA-cTAR DNA duplex is indicated by TAR*–cTAR. In the absence of cTAR and NC (lanes 2), the band (TAR*–TAR*) above the monomer probably corresponds to the dimeric form of TAR described elsewhere (Andersen et al. 2004). (B) The graph was derived from the experiments shown in A. Filled circles, in the absence of NC; filled triangle, NC (1.5 µM); open triangles, NC (3 µM); open circles, NC (6 µM).