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. 2016 Apr;22(4):583–596. doi: 10.1261/rna.054064.115

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© 2016 Lamichhane et al.; Published by Cold Spring Harbor Laboratory Press for the RNA Society

This article is distributed exclusively by the RNA Society for the first 12 months after the full-issue publication date (see http://rnajournal.cshlp.org/site/misc/terms.xhtml). After 12 months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.

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FIGURE 2. — Ectopic genes encoding cy-tRNAs rescue the metabolic phenotypes of tit1-Δ cells. (A–C) Growth efficiency of various S. pombe cells transformed with empty vector (ev), Tit1⁺, or the genes for tRNAs as indicated to the left (see text) were monitored. This was done by spotting serial dilutions onto EMM plates with glucose (A), or with glucose and containing rapamycin at 50 ng/mL (B), or glycerol as the carbon source (C). +5 tRNAs = one gene each for tRNA^SerAGA, tRNA^SerUGA, tRNA^SerCGA, tRNA^Tyr, and tRNA^Trp on the same plasmid; +3 tRNAs Ser = one each for tRNA^SerAGA, tRNA^SerUGA, tRNA^SerCGA; +2X-Ser-AGA = two genes for tRNA^SerAGA. (D) The cells were also spotted onto EMM with limiting amount of adenine (10 mg/L) to reveal suppressor-tRNA-mediated opal suppression of ade6-704 in the same cells (see text).