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. 2016 Mar 16;6:22868. doi: 10.1038/srep22868

Table 1. Human oligonucleotide primers for qRT-PCR.

Gene Names Sequences (5′→3″) Product size (bp) Ta (°C)
Oct4 F- CGTGAAGCTGGAGAAGGAGAAGCTGR- CAAGGGCCGCAGCTTACACATGTTC 247 66
SOX2 F- GCACATGAACGGCTGGAGCAACGR- TGCTGCGAGTAGGACATGCTGTAGG 207 66
NANOG F- ACCTTGGCTGCCGTCTCTGGR- AGCAAAGCCTCCCAATCCCAAACA 151 64
KLF4 F- GCAGCCACCTGGCGAGTCTGR- CCGCCAGCGGTTATTCGGGG 130 65
RUNX2 F- CCAAATTTGCCTAACCAGAAR- GCTCGATTGCAATTGTCTCT 335 56
OCN F- CCCAGGCGCTACCTGTATCAAR- CTGGAGAGGAGCAGAACTGG 209 61
SPARC F- GCTCCACCTGGACTACATCGR- GGAGAGGTACCCGTCAATGG 325 59
VEGFa F- CTACCTCCACCATGCCAAGTR- GCAGTAGCTGCGCTGATAGA 109 59
bFGF F- GGCTATGAAGGAAGATGGAAGATTR- TGCCACATACCAACTGGTGTATTT 130 62
GAPDH F- CCACTCCTCCACCTTTGACR- ACCCTGTTGCTGTAGCCA 102 60