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. 2016 Mar 16;6:23259. doi: 10.1038/srep23259

Table 1. Gene-specific primers used for gene knock-out.

Primers Sequence(5′-3′) Application
dmtA/P1 CCAGCAGCATCTTGACCTC dmtA deletion and probe
dmtA/P3 GGGTGAAGAGCATTGTTTGAGGCAACACGATGGTAGGGAACTG
dmtA/P4 GCATCAGTGCCTCCTCTCAGACCCTCTTTCGGACCTTGCG
dmtA/P6 CAGCAGTCATTGTAATCCAGCC
dmtA/P2 TTCAAGGCTTCCAATCCACAG
dmtA/P5 AGAGGCAAGGGAGAAGAAGC
PyrG/F GCCTCAAACAATGCTCTTCACCC dmtA deletion
PyrG/R GTCTGAGAGGAGGCACTGATGC
dmtA/UA AGCCTCGGCATTCGCACTG dmtA mutant screen
P801/R CAGGAGTTCTCGGGTTGTCG
dmtA/OF TAGTGAATCCCAACCACCTTT dmtA mutant screen
dmtA/OR CGTTCGCACCCTCTATTGTAT
dmtA-GFP/F CCTCGAGGTCGACGGTATCGATGCATCGAATATGAGATCAGGTC GFP fusion
dmtA-GFP/R CTGCAGGCATGCAAGCTTATCGATCCCAGAAGCAATGACGAGG