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. 2016 Mar 16;6:23135. doi: 10.1038/srep23135

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(A,B) NOX1 and p67^phox expression was analyzed by IHC in TRAMP prostate tissue with different stages of the disease as well as in non-transgenic mice prostate tissue as negative control. Representative photographs are presented at 100x; inset represent further magnification of a part of the photograph. Immunoreactivity (represented by brown staining) of NOX1 and p67^phox was scored as described in methods. Abbreviations: NC: Negative control; LG: Low-grade; HG: High-grade; PIN: Prostate intra-epithelial neoplasia; WD: Well differentiated; MD: Moderately differentiated; PD: Poorly differentiated. *p ≤ 0.001.