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. 2016 Mar 14;60:10.3402/fnr.v60.30932. doi: 10.3402/fnr.v60.30932

Table 3.

The content of total flavonoids in silkworm cocoons by the two methods (mg/g ±SD)

Daizo White Colored cocoons



Methods Aglycones Cocoon Cocoon 1201 1-342 1-425 1-427 1-451 1-474 1-901 1-949 4-524
HAE-HPLC Quercetin 1.98 0.02 0.04 1.41 0.81 2.78 0.04 0.04 0.22 2.38 1.57
(Q) ±0.06 ±0.01 ±0.01 ±0.08 ±0.02 ±0.33 ±0.03 ±0.01 ±0.01 ±0.12 ±0.13
Kaempferol 0.42 0 0.02 0.22 0.18 0.44 0.02 0.03 0.06 0.37 0.33
(K) ±0.01 0 ±0.01 ±0.01 ±0.02 ±0.04 ±0.01 ±0.01 ±0.01 ±0.02 ±0.01
Total sum 2.40 0.02 0.05 1.63 0.99 3.22 0.06 0.07 0.28 2.75 1.89
(Q + K) ±0.07 ±0.01 ±0.01 ±0.08 ±0.03 ±0.31 ±0.01 ±0.01 ±0.01 ±0.11 ±0.12
Colorimetry Total flavonoids 2.59 0.48 0.43 2.79 3.23 2.06 1.01 0.69 2.07 2.90 1.07
(Rutin) ±0.09 ±0.12 ±0.25 ±0.19 ±0.37 ±0.22 ±0.18 ±0.10 ±0.20 ±0.01 ±0.1
1.28 0.24 0.21 1.38 1.60 1.02 0.50 0.34 1.02 1.44 0.53
Quercetin ±0.04 ±0.06 ±0.12 ±0.09 ±0.18 ±0.11 ±0.09 ±0.05 ±0.10 ±0.01 ±0.05

Q + K is quercetin + kaempferol, ‘≈Quercetin’ means that the value of total flavonoids by the UV colorimetric method was converted into an equivalent quantity of quercetin according to the molecular mass of rutin.