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. 2016 Mar 16;6:23082. doi: 10.1038/srep23082

Figure 3. Generation of StRDR1-depleted transgenic potato lines.

Figure 3

PCR of plant genomic DNA to verify the presence of the StRDR1-specific hairpin constructs RNAi1 (a) or RNAi2 (b) in the transformed potato lines. PCR used a primer complementary to a sequence located in the 35S promoter of the T-DNA insert and primers specific to either RNAi 1 and 2, to amplify regions of 535 bp and 550 bp, respectively. DNA from non-transformed (NT) plants and a no template PCR control (negative: -ve) were included in the analysis and control PCR reactions were carried out using primers specific for β-tubulin (expected product of 480 bp). (c) StRDR1 accumulation is efficiently decreased in a potato lines expressing the RNAi1 (Line 1.3) and RNAi2 construct (Line 2.5) (for other examples see Supplementary Fig. S3). Accumulation levels of transcripts of StRDR2 (d) and StRDR6 (e) were not diminished in plants of transgenic lines expressing either RNAi1 or RNAi2 to inhibit StRDR1 expression. Error bars represent standard error of the mean.