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Apoptotic cells were identified by TUNEL. The corresponding images were merged. (A–C) Control group. (A′–C′) Group treated with 5 μM coagulansin-A. Scale bar, 100 μm. Apoptotic cells are indicated by yellow arrows. The graph shows the number of apoptotic cells per embryo. Columns with different superscripts are significantly different (P<0.05).
