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. 2016 Mar 14;4:e1792. doi: 10.7717/peerj.1792

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© 2016 Zhang et al.

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ) and either DOI or URL of the article must be cited.

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RPS15A knockdown induced apoptotic cells were determined by flow cytometry analysis after Annexin V-APC staining. (A, B) Histograms of FACS analysis. (C, D) Percentage of apoptotic cells. **P < 0.01 versus Lv-shCon. (E) Key factors of P53 signaling pathway, such as P21, TP53I3 and SESN2, were examined in Lv-shRPS15A infected A549 cells by using western blotting method. The protein level of GAPDH was employed as a control.