Table 3. miRNA biogenesis proteins of Mytilus galloprovincialis.
Protein name, GenBank ID, transcript (bp) and protein length (aa), identified domains and annotation (first hit, e-value and percentage of similarity) are reported.
| Protein name | GenBank ID | Transcript length (bp) | Protein length (aa) | Identified domain(s) | Annotation (first hit) | E-value (e∧) | Similarity (%) | |
|---|---|---|---|---|---|---|---|---|
| Key miRNA biogenesis proteins | MgDROSHA | KT447251 | 4,384 | 1,377 | 2× RIBO III + 1× DSRM | Ribonuclease 3-like (Crassostrea gigas) | 0 | 67 |
| MgDGCR8 | KT447252 | 2,483 | 728 | 1× WW + 2× DSRM | Microprocessor complex subunit DGCR8-like (Crassostrea gigas) | 0 | 50 | |
| MgXPO5 | KT447259 | 3,875 | 1,201 | XPO1 | Exportin-5-like (Crassostrea gigas) | 0 | 55 | |
| MgRAN | KT447254 | 1,113 | 214 | RAN | GTP-binding nuclear protein Ran (Crassostrea gigas) | −143 | 93 | |
| MgDICER | KT447258 | 6,013 | 1,850 | PDB + Helicase + DICER + PAZ + 2× RIBO + DSRM | Endoribonuclease Dicer-like (Crassostrea gigas) | 0 | 58 | |
| MgTARBP2 | KT447253 | 7,583 | 321 | 3× DSRM | Probable RISC-loading complex subunit (Crassostrea gigas) | −143 | 69 | |
| MgAGO | KT447257 | 3,337 | 892 | DUF + PAZ + PIWI | Protein argonaute-2-like (Crassostrea gigas) | 0 | 84 | |
| MgPIWIa | KT447255 | 2,686 | 867 | PAZ + PIWI | Piwi-like protein 1 (Crassostrea gigas) | 0 | 75 | |
| MgPIWIb | KT447256 | 3,603 | 948 | PAZ + PIWI | Piwi-like protein 2 (Hydra vulgaris) | 0 | 59 | |
| Other interacting proteins | MgGW182 | KT447250 | 3,825 | 1,274 | UBA + RRM | Trinucleotide repeat-containing gene 6C protein-like (Crassostrea gigas) | 0 | 45 |
| MgCNOT1 | KT694355 | 5,373 | 1,791 | DUF3819 + NOT1 | CCR4-NOT transcription complex subunit 1-like (Crassostrea gigas) | 0 | 69 | |
| MgCNOT2 | KT694357 | 864 | 288 | NOT2_ 3_ 5 | CCR4-NOT transcription complex subunit 2 (Pinctada fucata) | −156 | 82 | |
| MgCNOT3 | KT694358 | 2,142 | 714 | NOT3 + NOT2_ 3_ 5 | CCR4-NOT transcription complex subunit 3-like (Crassostrea gigas) | 0 | 97 | |
| MgCNOT6 | KT694359 | 2,592 | 864 | Exo_ endo_ phos | Uncharacterized protein LOC105348954 isoform X1 (Crassostrea gigas) | 0 | 71 | |
| MgCNOT7 | KT694360 | 897 | 299 | CAF1 | CCR4-NOT transcription complex subunit 7-like (Crassostrea gigas) | 0 | 84 | |
| MgCNOT9 | KT694361 | 927 | 309 | RCD1 | Cell differentiation protein RCD1 homolog (Crassostrea gigas) | 0 | 93 | |
| MgCNOT10 | KT694356 | 2,133 | 711 | TPR_ 1 | CCR4-NOT transcription complex subunit 10-like (Crassostrea gigas) | 0 | 71 | |
| MgDDX5 | KT694371 | 1,740 | 538 | DEAD + Helic | ATP-dependent RNA helicase DDX5 (Crassostrea gigas) | 0 | 75 | |
| MgDDX6 | KT694372 | 1,332 | 443 | DEAD + Helic | ATP-dependent RNA helicase me31b (Crassostrea gigas) | 0 | 88 | |
| MgDDX20 | KT694373 | 1,836 | 612 | DEAD + Helic | ATP-dependent RNA helicase DDX20 (Crassostrea gigas) | 0 | 77 | |
| MgDDX42 | KT694374 | 2,196 | 731 | DEAD + Helic | ATP-dependent RNA helicase DDX42 (Crassostrea gigas) | 0 | 72 | |
| MgPABP | KT694365 | 1,881 | 627 | 4× RRM + PABP | polyadenylate-binding protein 4 (Hydra vulgaris) | 0 | 74 | |
| MgeIF4G | KT694364 | 5,019 | 1,672 | MIF4G + MA3 + W2 | eukaryotic translation initiation factor 4 gamma (Crassostrea gigas) | 0 | 57 | |
| MgPAN2 | KT694367 | 3,606 | 1,202 | UCH_ 1 + RNase_T | PAB-dependent poly(A)-specific ribonuclease subunit PAN2 (Lingula anatina) | 0 | 72 | |
| MgPAN3 | KT694368 | 2,334 | 778 | None | PAB-dependent poly(A)-specific ribonuclease subunit PAN3 (Lingula anatina) | 0 | 67 | |
| MgDCP1 | KT694362 | 1,611 | 536 | DCP1 | mRNA-decapping enzyme 1A-like (Crassostrea gigas) | −126 | 73 | |
| MgDCP2 | KT694363 | 1,313 | 385 | DCP2 + NUDIX | m7GpppN-mRNA hydrolase (Lingula anatina) | −117 | 67 | |
| MgPRMT5 | KT694369 | 1,893 | 631 | PRMT5 | protein arginine N-methyltransferase 5-like (Crassostrea gigas) | 0 | 72 | |
| MgTudor-11 | KT694370 | 2,682 | 894 | 4× SNc + TUDOR | Hypothetical protein mRNA (Lottia gigantea) | 0 | 73 | |
| MgMaelstrom | KT694366 | 1,321 | 404 | HMG + MAEL | Protein maelstrom (Crassostrea gigas) | −155 | 62 |