Table 1.

HA antibody levels (EU) and hemagglutination inhibition (HI) response in mice

		G.M. IgG anti-HA		No. with
Immunogen	Dosage	EU (25-75 centiles)		HI ≥40
rHA-alum	5 μg × 2	14.4	(10.6-21.2)	8/10
rHA-alum	5 μg × 3	177.8	(112-286)	8/10
rHA-formalin	5 μg × 2	26.6	(22.2-41.3)	8/10
rHA-formalin	5 μg × 3	40.1	(33.5-52.2	10/10
rHA-formalin-alum	5 μg × 2	14.4	(10.9-20.1)	3/10
rHA-formalin-alum	5 μg × 3	212.7	(119.3-270.8)	10/10
rHA-alum-formalin	5 μg × 2	3.2	(1.7-9.15)	5/10
rHA-alum-formalin	5 μg × 3	10.0	(7.95-17.1)	9/10
rHA	5 μg × 3	61.5	(24.7-249)	9/9
rHA-alum	15 μg × 3	449.4	(224-757)	10/10
PBS (control)		0.01	(0.005-0.005)	0/10

Mice, 5-6 week old, were injected s.c. 2 or 3 times 2 weeks apart and bled 1 week after the last injection.

14.4 vs 177.8, 212.7 p<0.0001; 26.6 vs 40.1 p Not Significant (NS);

3.2 vs 10 p NS; 177.8 vs 40.1, 10 p<0.0001; 177.8 vs 212.7 p NS;

449.4 vs 177.8, 212.7 p<0.01