Fig. 7.
Microglia but not Ly6Chi monocytes are located in the vicinity of Aβ plaques. a-d show immunolabeled coronal sections from T. gondii infected 5xFAD mice. a, b Co-labeling of Iba1 (microglia) and Aβ (plaques) reveals close interaction of microglia with plaques. a Low magnification (20x) overview of the cortex. Scale bar, 200 μm. b Representative cortical plaque surrounded by microglial processes. 63x magnification, scale bar, 20 μm. c, d In sections co-labeled for Ly6C (Ly6Chi monocytes) and Aβ (plaques), Ly6Chi monocytes were not located in the direct vicinity of plaques. c Low magnification (20x) overview of the cortex. Scale bar, 200 μm. d Representative cortical plaque with a nearby Ly6C+ blood vessel but no associated Ly6Chi monocytes. 63x magnification, scale bar, 20 μm. e, f The sections were analyzed to quantify the fluorescence intensity of (e) Iba1 and (f) Ly6C in the direct environment of plaques. The black line represents the average at a given distance. AU, arbitrary units