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. 2016 Feb 24;16:89. doi: 10.1186/s12879-016-1433-2

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© Siriyasatien et al. 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — PCR amplification of the ITS1 gene against saliva, buffy coat, blood, and tissue samples of nodular leishmaniasis case (a) and asymptomatic case (b). PCR amplicons were analyzed by electrophoresis on a 1.5 % agarose gel and stained with ethidium bromide. Lane S1, B1 and BF1: first saliva, blood and buffy coat collection, respectively; lane S2, B2 and BF2:second saliva, blood and buffy coat collection, respectively; and lane S3,B3 and BF3: third saliva, blood and buffy coat collection, respectively; T: tissue, lane M: molecular mass marker (100 basepairs [bp]); lane P: positive control containing extracted DNA from cultured L. martiniquensis-produced fragments of 379 bp, lane N: negative control (no DNA template: double-distilled water); lanes N1–N3: negative control (DNA template from non-infected saliva, blood, and buffy coat, respectively); and a PCR for template DNA control shown below (628 bp)