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. Author manuscript; available in PMC: 2016 Mar 16.
Published in final edited form as: J Mol Biol. 2010 Aug 13;401(2):309–322. doi: 10.1016/j.jmb.2010.05.058

Fig. 4.

Fig. 4

The effect(s) of ethidium bromide on the efficiency of capsid assembly. Capsid assembly was monitored at increasing stoichiometric ratios of CP2:5′RNA in the presence and in the absence of 30 μM EtBr. (a) Using native agarose gel electrophoresis, no signal corresponding to MS2 capsids was detected in the presence of EtBr after 4 h. (b) Sedimentation velocity analysis confirms that assembly is restricted in the presence of EtBr. 5′ RNA sediments slower in the presence of EtBr, suggesting that EtBr intercalation results in expansion of RNA structure and inhibits folding of the RNA into a structure compatible with the capsid.