Table 1.
S exp | S 20,w | f/f0 | R S | |
---|---|---|---|---|
MS2 virion | 74.6±0.5 | 75.1 | 1.1 | 11.6 |
MS2 capsids | 65.4±2.0 | 65.8 | 1.2 | 11.7 |
CP2 | 2.3±0.2 | 2.3 | 1.4 | 2.8 |
Virion RNA | 25.7±1.5 | 25.8 | 3.0 | 18.4 |
Virion RNA + EDTA | 22.1±1.8 | 22.3 | 3.4 | 21.3 |
5′RNA | 20.4±1.3 | 20.5 | 2.9 | 15.9 |
5′RNA + EDTA | 17.3±1.2 | 17.4 | 3.4 | 18.4 |
3′RNA | 21.2±1.3 | 21.3 | 2.9 | 16.1 |
3′RNA + EDTA | 18.5±1.6 | 18.6 | 3.5 | 19.5 |
iRNA | 9.7±0.9 | 9.8 | 3.2 | 12.8 |
iRNA + EDTA | 10.6±1.8 | 10.7 | 3.0 | 11.8 |
Sexp is the experimental sedimentation coefficient in 40 mM ammonium acetate, 1 mM magnesium acetate at 20 °C.
S20,w is the sedimentation coefficient corrected for buffer conditions. f/f0 The frictional coefficient, an indication of how spherical a particle is. f/f0 is 1 for a spherical particle.
RS=Stokes radius.