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. 2016 Mar 15;9:15. doi: 10.1186/s13048-016-0225-z

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© Maekawa et al. 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — mRNA expression of EZH2, SETDB2, HDAC4, HDAC10, and CIITA in rat granulosa cells undergoing luteinization after ovulation induction. Granulosa cells were obtained from rats treated with eCG before (0 h), 4, and 12 h after hCG injection. EZH2, SETDB2, HDAC4, HDAC10, and CIITA were identified as genes related to histone acetylation or histone methylation, and validated with additional samples (n = 5 in each time point) by real-time RT-PCR. The value of mRNA of each gene was normalized to that of an internal control (GADPH). Data were expressed as a ratio of mRNA of each gene to GADPH. Each bar represents the mean +/− SEM of five animals. a; p < 0.01 vs. 0 h, b; p < 0.05 vs. 0 h