. Author manuscript; available in PMC: 2016 Jul 21.
Published in final edited form as: Nat Protoc. 2016 Jan 21;11(2):347–358. doi: 10.1038/nprot.2016.006
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| Step | Temperature | Time |
|---|---|---|
| 1 | 25°C | 5 min |
| 2 | 42°C | 60 min |
| 3 | 70°C | 15 min |