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. Author manuscript; available in PMC: 2016 Mar 16.
Published in final edited form as: Cell Metab. 2009 Feb;9(2):140–151. doi: 10.1016/j.cmet.2008.12.005

Figure 7. Glucagon content and secretion in Rip-DTG diabetic mice.

Figure 7

(A) Plasma glucagon and glucagon content per islet from WT, Rip-DTG and syngeneically transplanted Pdx-DTG mice determined at ~3.5 months of age (mean ± s.e.m., n= 3 animals in each case). Plasma glucagon and glucagon content were determined by radioimmunoassay (see Experimental Procedures). Significant differences *p<0.05 between test and Wt are indicated. (B) Glucose-dependent glucagon secretion from isolated islets as in A. Batches of 10 islets were statically incubated at different glucose concentrations for 1 hour. Glucagon release was measured in the media by radioimmunoassay (n=3 mice in each group, samples done in triplicates). Significant differences *p<0.05 between test and same glucose concentration from controls are indicated.