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. 2016 Mar 16;9:154. doi: 10.1186/s13071-016-1437-1

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© Gómez-Morales et al. 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — Assay validation study: optical density and cut-off values of dog/fox serum samples by ELISA. Serum samples from presumably Trichinella spp.-free (n = 598) and Trichinella spp.-infected (n = 21) dogs/foxes were tested by ELISA using Trichinella spiralis and Toxocara canis excretory/secretory antigens. Out of the 619 sera, 47 tested positive for T. spiralis; of these, 26 sera from presumably Trichinella spp.-free dogs tested negative by Western blot (Wb), and 21 sera from Trichinella spp.-infected dogs/foxes tested positive by Wb (see Table 1). Out of the 619 sera, 233 (37.6 %) tested positive by ELISA for T. canis (see Table 1)