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. 2016 Mar 16;9:154. doi: 10.1186/s13071-016-1437-1

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© Gómez-Morales et al. 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — Assay validation study: western blot (Wb) and SDS-PAGE of excretory/secretory antigens from Trichinella spiralis (T_ESA) and from Toxocara canis (Tox_ESA). a. Lanes 1 and 2, T_ESA blotted with two serum samples from T. spiralis infected dogs. Lane 3, T_ESA blotted with a serum sample from a Trichinella-free dog. Lanes 4 and 5, T_ESA blotted with the serum samples from two T. canis infected dogs. Lane 6, Tox_ESA blotted with a serum sample from a T. canis uninfected dog; Lane 7, Tox_ESA blotted with a serum sample from a T. canis infected dog; Mw, molecular weight markers. b. Ten percent SDS-PAGE of T_ESA (Lane 1) and Tox_ESA (Lane 2) stained with Coomassie Blue; Mw, molecular weight markers