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. 2016 Mar 16;14:75. doi: 10.1186/s12967-016-0832-x

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© Sun et al. 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 6 — Regulation of the expression of B2M at mRNA and protein levels by the TGF-β signaling pathway in ovarian cancer cell lines. OVCAR-3 and SK-OV-3 cells were pre-treated with a TGF-β type I receptor kinases inhibitor (SB431542, 10 μM) for 30 min and then treated with 10 ng/ml TGF-β1. B2M mRNA in OVCAR-3 (a) and SK-OV-3 (b) cells was detected by quantitative RT-PCR using primers specific to B2M after TGF-β1 treatment for 24 h. B2M protein in OVCAR-3 (c) and SK-OV-3 (d) cells was detected by Western blot analysis using a specific antibody against B2M and β-actin after TGF-β1 treatment for 72 and 48 h, respectively. Histograms show the quantitative analysis of the gels after densitometry. The results are representative of three independent experiments. *P < 0.05