Table 2.
Morphometric parameters of body weight and heart and tumour tissues
| Groups | C | W | L | LW |
|---|---|---|---|---|
| Initial body weight (g) | 401.6 + 9.1 | 413.4 + 5.93 | 417.7 + 8.08 | 402.5 + 7.35 |
| Final body weight (g) a | 454.6 + 10.1 | 397.6 + 17.7* | 476.2.9 + 10.9 | 396.1 + 12.15*# |
| Percentage of final body weight (%) a § | 113.3 + 1.0 | 95.9 + 3.4* | 113.9 + 1.1 | 98.5 + 2.5*# |
| Weight gain (g) per day a § | 2.52 + 0.21 | −0.75 + 0.67* | 2.78 + 0.23 | −0.31 + 0.49*# |
| Heart weight (grams) b | 1.27 + 0.05 | 1.14 + 0.04* | 1.42 + 0.06* | 1.15 + 0.06*# |
| Relative heart weight (%) b §, § | 0.28 + 0.01 | 0.30 + 0.01* | 0.29 + 0.01 | 0.32 + 0.01*# |
| Myocardial protein (µg/mg) c | 88.65 + 6.89 | 80.84 + 3.51* | 112.20 + 6.07* | 89.75 + 7.58 |
| Heart water content §§§ | 73.33 + 2.91 | 67.54 + 4.02 | 71.37 + 3.10 | 71.84 + 4.05 |
| Left ventricular thickness (µm) d | 281.7+ 12.4 | 221.5+ 9.1*# | 323.8+ 16.8* | 186.5+ 5.9*# |
| Right ventricular thickness (µm) e | 83.9+ 6.2 | 88.9+ 4.2 | 101.2+ 5.3* | 66.4+ 4.6*# |
| Tumour weight (g) | — | 55.66 + 5.98 | — | 48.84 + 7.40 |
| Relative tumour weight (%)f | — | 14.86 + 2.08 | — | 13.97 + 2.14 |
Legend: C (Control group), W (untreated tumour‐bearing rats group), L (leucine‐rich diet group), LW (tumour‐bearing rats fed a leucine‐rich diet).
(Final body weight/Initial body weight) × 100%; weight gain = (final body weight − initial body weight) / days.
(Heart weight/body weight) × 100%.
(Fresh heart weight − dry heart weight) / fresh heart weight × 100%. Decreased body weight, and percentage/weight gain produced by tumour effect.
(Two‐way ANOVA, P < 0.0001; followed by the Bonferroni test *P < 0.05 vs. control group, # P < 0.05 vs. leucine group; n = 5). Decreased heart weight and relative heart weigh produced by tumour effect.
(Two‐way ANOVA, F = 10.45 P = 0.0019; followed by the Bonferroni test *P < 0.05 vs. control group, # P < 0.05 vs. leucine group; n = 5).
Myocardial protein content changed mainly by diet effect (two‐way ANOVA, F = 6.93 P = 0.0129; followed the Bonferroni test * P < 0.05 vs. control group, n = 5) and by tumour effect (two‐way ANOVA, F = 6.03 P = 0.0197; followed by the Bonferroni test * P < 0.05 vs. control group, n = 5).
Left ventricle wall reduction as a tumour effect (two‐way ANOVA, F = 70.63 P < 0.0001; followed by the Bonferroni test * P < 0.05 vs. control group, # P < 0.05 vs. leucine group; n = 5).
Right ventricle wall reduction as an interaction effect between tumour and diet (two‐way ANOVA, F = 14.98 P = 0.0005; followed by the Bonferroni test * P < 0.05 vs. control group, # P < 0.05 vs. leucine group; n = 5).
(Tumour weight/final body weight without tumour) × 100%.