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. Author manuscript; available in PMC: 2016 Mar 16.
Published in final edited form as: Methods Enzymol. 2015 May 16;559:99–110. doi: 10.1016/bs.mie.2014.11.010
Component Final concentration Stock Amount
2× Buffer G 1× 2× 25 ml
Triton X-100 0.5% 20% 1.25 ml
Glycerol 10% 50% 10 ml
DTT 0.5 mM 1 M 25 µl
PMSF 1 mM 100 mM 500 µl
Protease Inhibitor Cocktail 1 tablet

Add water to 50 ml