Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2016 Mar 16;11(3):e0151359. doi: 10.1371/journal.pone.0151359

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2016 Di Sanzo et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

PMC Copyright notice

Fig 1 — Panel A. Alignment of the sequences of the FHC mRNA and the FTH1P3 pseudogene from nt. 271 to nt. 720. The FHC accession number is NM_002032.2. The FTH1P3 accession number is NR_002201.1. The ΨF, ΨR, HF and HR oligonucleotide sequences are boxed. Panel B. PCR analysis of FTH1P3 transcript, performed with ΨF and ΨR oligonucleotides. Lane 1: genomic DNA from MCF-7 cells; lane 2: DNaseI-treated total RNA from MCF-7 cells; lane 3: cDNA from MCF-7 cells; lane 4: negative control without template (NC). The predicted PCR product, indicated by an arrow, is 87-bp long. DNA sequencing electropherogram (nt. 38 to nt. 63) of the FTH1P3 fragment. Panel C. PCR analysis of FTH1P3 transcript, performed with HF/ΨR (lane 1), negative control without template (lane 2). The predicted PCR product, indicated by arrow is 260-bp long. DNA sequencing electropherogram (nt. 139 to nt. 173) of the 260-bp FTH1P3 fragment. Panel D. PCR analysis of FTH1P3 transcript elongated with ΨF/HR oligonucleotides (lane 1), negative control without template (lane 2). The predicted PCR products, indicated by arrows, are 149-bp for FTH1P3 and 117-bp for FHC. DNA sequencing electropherogram (nt. 20 to nt. 54) of the 149-bp FTH1P3 fragment and of the 117-bp FHC fragment (nt. 35 to nt. 55).