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. 2016 Mar 16;11(3):e0151128. doi: 10.1371/journal.pone.0151128

Fig 2. Cytotoxicity of DnBP determined by flow cytometry assays.

Fig 2

(a) coelomocyte apoptosis (a1,a2) stained with Annexin V-FITC and PI (Upper left quadrant, necrotic cells; upper right quadrant, late/secondary apoptotic cells; bottom left quadrant, live cells; and bottom right quadrant, early/primary apoptotic cells) in the 28th day and (b) lysosomal membrane stability characterized by NRR time in extruded coelomocyte of treated E. fetida in the 7th, 14th, 21st and 28th day after exposure in spiked natural soil CK, B-1, B-2, B-3, and B-4 for 28 days (n = 4; error bars, SEM). Refer other annotates to Fig 1.