Fig 2. Time-dependent inhibition of genes in FRTL-5 cells treated with BPA verified by qRT-PCR.

qRT-PCR analysis of some microarray down-regulated genes in FRTL-5 cells after 1-, 3-, and 7-day 10⁻⁹ M BPA treatment. In (A) and (B), genes enriched in the “DNA replication, recombination, and repair, developmental disorder, hereditary disorder” network, predicted in the 7-day gene set, are reported. In (C) and (D), transcripts leading to the prediction of p53 inhibition are shown. Data are reported as the ratio between mRNA content in BPA-treated and control samples. The mean ± standard deviation of three independent experiments is reported. *p-value <0.05; **p-value <0.01.