Fig 4. ASS1 protein is expressed in mesothelioma samples and upregulated when compared to normal tissues.

(A) ASS1 stain intensity was measured in 88 mesothelioma samples by immunohistochemistry (each tumor in replicates of 3) in a semi-quantitative fashion as outlined in the Methods section. Only 8 samples out of 88 (10%) showed no ASS1 staining (B) ASS1 stain intensity was also measured in another 88 mesotheliomas with their paired 88 normal tissues. Analysis of matched pairs (tumor vs normal tissue control obtained from the same patients) of the 88 patients showed that ASS1 expression was significantly higher in mesothelioma (* p < 0.0001, n = 88 matched pairs).