Fig 2. Dissection of the MamO catalytic triad.
(A) Magnetic response of cultures with the indicated mamO alleles. The coefficient of magnetism is an optical density-based method assessing cells’ ability to turn in a magnetic field. Biological replicates represent independent cultures of each strain. Each measurement represents the average and standard deviation from three independent experiments. (B) Transmission electron microscopy (TEM) of whole AMB-1 cells with the indicated genetic backgrounds. (C) Magnetite crystals from cells with the indicated mamO alleles. (D) Crystal size distributions for the indicated mamO alleles as assessed by TEM. n > 200 for each strain.