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. 2016 Mar 16;11(3):e0151534. doi: 10.1371/journal.pone.0151534

Fig 1. Cell viability evaluation of human primary osteoblasts in mono-culture.

Fig 1

A) Metabolic activity of human primary osteoblasts (hOB) incubated for 2 and 7 days on Ti6Al4V, PALACOS® R, and PALACOS® R+G+V, and measured using the WST-1 assay. Values are given as percentage of the polystyrene control. PALACOS® R+G+V displayed lower cell activity than the other materials on both days. (*) Denotes significance with respect to the polystyrene control at each time point, and (+) significance with respect to the same material on day 2 (n = 4, mean ± SEM). B) Live/dead staining of the hOB cultured in a monolayer on the test samples after 2 and 7 days of incubation. All images were taken at 40x magnification. Scale bars are 200 μm.