Table 2.
Kinetic Parameters for TcGlcK and HsHxKIV.
| Enzyme | Constructd | KM (mM) | kcat (min−1) | kcat/KM (mM−1 min−1) |
|---|---|---|---|---|
| His6-TcGlcKa | pET-TcGlcK-xtale | 0.948 ± 0.295 | (4.67 ± 2.15) × 102 | (4.93 ± 2.26) × 102 |
| His6-TcGlcKb | NNAf | 1.00 ± 0.06 | 8.95 × 104 | 8.95 × 104 |
| His6-HsHxKIVa | pET-HsHxKIV-xtale | 41.8 ± 8.7 | (1.30 ± 1.73) × 104 | (3.11 ± 4.14) × 102 |
| wt-HsHxKIVc | pEhgk-WT | 6.0 ± 0.4 | 3.98 × 103 | 6.63 × 102 |
a
From this study; assay was performed at pH 7.6 and 22 °C; measurements performed in triplicate.
b
From ref (28); assay was performed at pH 8.5 and room temperature.
c
From ref (46); wild-type human pancreatic β-cell hexokinase IV; assay was performed at pH 7.5 and 30 °C.
d
Refer to corresponding references for construct details.
e
Extension “-xtal” signifies that the construct has a custom design to allow the enzyme to be suitable for X-ray crystallography.
f
Name not available.