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. Author manuscript; available in PMC: 2017 Mar 15.
Published in final edited form as: Immunity. 2016 Feb 23;44(3):582–596. doi: 10.1016/j.immuni.2016.01.001

Figure 1. Akt1 activation in alveolar macrophages is associated with pulmonary fibrosis.

Figure 1

(A) Immunoblot and (B) densitometric analysis of alveolar macrophages isolated by BAL from normal subjects (n = 4) and IPF patients (n = 5), Student’s t-test. (C) Quantitative analysis and representative immunoblot (Inset) of alveolar macrophages isolated by BAL from WT (n =5) and Akt1+/− mice (n =5) exposed to bleomycin (2 U/kg) intratracheally, Student’s t-test. After bleomycin exposure, mice were euthanized 21 days later and lungs from (D) WT and (E) Akt1+/− mice were removed and processed for Masson’s trichrome staining. Representative micrographs from 1 of 6 mice are shown. Bar, 600 μm. (F) Hydroxyproline assay of lungs removed from WT (n = 5) and Akt1+/− mice (n = 6) after bleomycin exposure, Student’s t-test. WT mice were exposed to saline (n = 4) or bleomycin (n = 5) intratracheally, BAL was performed 21 days later. (G) Immunoblot and (H) densitometry analysis. Student’s t-test. (I) Immunoblot analysis of macrophages isolated by BAL from WT (n = 4 saline n = 5 bleo) and Akt1−/−Lyz2-cre mice (n = 4 saline; n = 6 bleo). Excised lungs from WT and Akt1−/−Lyz2-cre mice exposed to (J) and (K) saline or (L) and (M) bleomycin were stained with Sirius red. Representative micrographs from WT (n = 4 saline; n = 5 bleo) and Akt1−/−Lyz2-cre mice (n = 4 saline; n = 7 bleo) are shown. Bar, 500 μm. (N) Hydroxyproline of lungs from WT (n = 4 saline; n = 5 bleo) and Akt1−/−Lyz2-cre mice (n = 4 saline; n = 6 bleo). One-way ANOVA with Tukey’s comparison. *, p < 0.05; **, p < 0.002; ***, p < 0.0001. A minumun of three independent experiments were conducted. Please see Figure S1.