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. Author manuscript; available in PMC: 2017 Mar 15.
Published in final edited form as: Cancer Res. 2015 Dec 30;76(6):1560–1568. doi: 10.1158/0008-5472.CAN-15-2401

Figure 5.

Figure 5

DDR1 inhibitor 7rh inhibits DDR1 phosphorylation and enhances cytotoxicity and protein synthesis inhibition by RG7787. A) KB or B) A431/H9 cells were treated with different concentrations of 7rh inhibitor for 3 hours and harvested for western blotting. Blots were probed using anti-phospho DDR1, anti-DDR1 or anti-Actin antibody. C–F) KB, A431/H9, HAY, and KLM1 cells, respectively, were treated with DDR1 inhibitor for 3 hours, followed by varying concentrations of RG7787. After 72 hours, cell viability was assessed by cell titer glow ATP assay. G–J) Protein synthesis was measured by radioactive leucine incorporation after 18 hours of RG7787 treatment. Each point represents a mean of four to six treated wells.