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. Author manuscript; available in PMC: 2017 Mar 15.
Published in final edited form as: Immunity. 2016 Mar 2;44(3):647–658. doi: 10.1016/j.immuni.2016.02.006

Figure 2. Spontaneous Systemic Dissemination of Gram-Negative Symbiotic Bacteria.

Figure 2

(A) qPCR of bacterial 16S rRNA gene in the spleens of SPF and GF WT mice. Each dot represents one mouse.

(B) Compositions of bacterial communities in mesenteric lymph nodes (MLNs), spleens (SPL), and fecal bacteria (FB) of same WT SPF mice.

(C) Abundance of gram-negative Enter-obacteriaceae, Porphyromondaceae, and Pre-votellaceae in MLNs, spleens, and feces from WT SPF mice. Each dot represents one mouse.

(D) Immunoblotting of bacterial antigens from fecal bacteria (FB), Escherichia coli (EC), Klebsiella pneumoniae (KP), Enterococcus faecalis (EF), and Clostridium bifermentans (CB) that were recognized by IgG in the serum of a 4-, 6-, or 10-week-old SPF WT mouse. A SDS-PAGE gel was separately stained with Coomassie blue to indicate total amounts of cell lysates loaded.

Data are representative of two to three independent experiments. Error bars indicate SD.

*p < 0.05, **p < 0.01, ***p < 0.001.

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