Table 1.
Summary of targets in the MRM assay based on LC-MS/MS.
| Target Codes | Targeted Proteins | Signature Peptides | Preca (m/z) |
Fragb (m/z) |
CEc (V) | RTd (min) |
|---|---|---|---|---|---|---|
| Albumin 1 | Albumin-1 | LVNEVTEFAK | 575.3 | 694.4 | 33.8 | 46 |
| Albumin 123 | Albumin-1/albumin-2/albumin-3 | AVMDDFAAFVEK | 671.8 | 171.1 | 38.6 | 93 |
| Albumin 3 (N) | Albumin-3 | EFNAETFTFHADTCTLSEK | 754.0 | 850.4 | 36.2 | 89 |
| a-1-antitrypsin 1 (N) | α-1-antitrypsin-1/ α-1-antitrypsin-2 |
TLNQPDSQLQLTTGNGLFLSEGLK | 858.8 | 963.5 | 40.8 | 98 |
| a-1-antitrypsin 2 | α-1-antitrypsin-2 | LSITGTYDLK | 555.8 | 201.1 | 32.8 | 55 |
| Apo E (N) | Apolipoprotein E | SWFEPLVEDMQR | 769.0 | 185.0 | 43.4 | 105 |
| Apo J 1 (N) | Apolipoprotein J-1 | ELDESLQVAER | 644.8 | 215.1 | 37.2 | 40 |
| Apo J 23 | Apolipoprotein J-2/ Apolipoprotein J-3 |
ASSIIDELFQDR | 697.3 | 922.4 | 39.9 | 106 |
| CC 3 | Complement component 3 | LVAYYTLIGASGQR | 756.4 | 902.5 | 42.8 | 75 |
| Fibrin b | Fibrin β | HQLYIDETVNSNIPTNLR | 709.7 | 827.5 | 34.2 | 63 |
| IG HC | Immunoglobulin heavy chain | NQVSLTCLVK | 581.3 | 820.5 | 34.1 | 56 |
| IG LC (N) | Immunoglobulin light chain | SGTASVVCLLNNFYPR | 899.4 | 923.5 | 50.0 | 112 |
| Plasminogen NT (N) | Plasminogen | WELCDIPR | 544.6 | 216.1 | 27.0 | 69 |
| Transthyretin 1 | Transthyretin-1 | TSESGELHGLTTEEEFVEGIYK | 819.1 | 189.1 | 39.0 | 90 |
| Transthyretin 12 (N) | Transthyretin-1/ Transthyretin-2 |
GSPAINVAVHVFR | 684.0 | 307.1 | 33.1 | 65 |
| VDBP (N) | Vitamin D-binding protein | VPTADLEDVLPLAEDITNILSK | 790.1 | 209.1 | 37.8 | 146 |
| CASA | Bovine α-casein (internal standard) | HQGLPQEVLNENLLR | 587.3 | 644.4 | 28.8 | 73 |
1
Precursor ion in a MRM transition;
b
Fragment ion in a MRM transition;
c
Collision energy for a MRM transition;
d
Retention time